Cover: Biochemistry: A Short Course, 5th Edition by Justin K. Hines; Catherine Reinke; John Tymoczko

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Biochemistry: A Short Course

Fifth  Edition©2025  Justin K. Hines; Catherine Reinke; John Tymoczko

About

Biochemistry: A Short Course connects the basic concepts of biochemistry to everyday life, in a presentation aimed at students planning careers in health care, pharmaceuticals, and other areas. You’ll explore how recent breakthroughs are reshaping our understanding of the fundamental principles in biochemistry and human health.

Table of Contents

Chapter 1 – Biochemical Similarities Demonstrate the Unity of Life
Chapter 2 – Water, Weak Interactions, and the Generation of Order out of Chaos
Chapter 3 – Amino Acids
Chapter 4 – Protein Three-Dimensional Structure
Chapter 5 – Protein Binding, Molecular Recognition, and Allostery
Chapter 6 – Techniques in Protein Biochemistry
Chapter 7 – Basic Concepts of Enzyme Action
Chapter 8 – Kinetics and Regulation
Chapter 9 – Mechanisms and Inhibitors
Chapter 10 – Carbohydrates
Chapter 11 – Lipids
Chapter 12 – Membrane Structure and Function
Chapter 13 – Signal-Transduction Pathways
Chapter 14 – Digestion: Turning a Meal into Cellular Biochemicals
Chapter 15 – Metabolism: Basic Concepts and Themes
Chapter 16 – Glycolysis
Chapter 17 – Gluconeogenesis
Chapter 18 – Preparation for the Citric Acid Cycle
Chapter 19 – The Citric Acid Cycle
Chapter 20 – The Electron-Transport Chain
Chapter 21 – The Proton-Motive Force
Chapter 22 – Phototrophy and the Light Reactions of Photosynthesis
Chapter 23 – The Calvin–Benson Cycle
Chapter 24 – Glycogen Degradation
Chapter 25 – Glycogen Synthesis
Chapter 26 – The Pentose Phosphate Pathway
Chapter 27 – Fatty Acid and Triacylglycerol Degradation
Chapter 28 – Fatty Acid Synthesis
Chapter 29 – Lipid Synthesis: Storage Lipids, Phospholipids, and Cholesterol
Chapter 30 – Protein Turnover and Amino Acid Catabolism
Chapter 31 – Amino Acid Synthesis
Chapter 32 – Nucleotide Metabolism
Chapter 33 – The Structure of Nucleic Acids: DNA and RNA
Chapter 34 – DNA Replication
Chapter 35 – DNA Repair and Recombination
Chapter 36 – RNA Synthesis and Regulation in Bacteria
Chapter 37 – Gene Expression in Eukaryotes
Chapter 38 – RNA Processing in Eukaryotes
Chapter 39 – The Genetic Code
Chapter 40 – The Mechanism of Protein Synthesis
Chapter 41 – Recombinant DNA Techniques

Justin Hines

Justin K. Hines is Professor of Chemistry at Lafayette College, where he teaches general chemistry and biochemistry courses and conducts education and NIH-funded laboratory research on protein misfolding with undergraduates. He received both his B.S. and PhD in Biochemistry from Iowa State University, where he studied the structure and regulation of the enzymes of central metabolism with Richard B. Honzatko and Herbert J. Fromm. He then completed a postdoctoral fellowship with Elizabeth A. Craig in Biochemistry at the University of Wisconsin–Madison. Professor Hines has won numerous awards for teaching and research, including being named a Cottrell Scholar by the Research Corporation for Science Advancement and a Henry-Dreyfus Teacher-Scholar by the Camille and Henry Dreyfus Foundation. He is also the author of the case-studies series for Macmillan’s three biochemistry textbooks. He enjoys running, hiking, games of any kind, and spending time with his wife and children.

Catherine Reinke

Catherine Reinke is an Associate Professor of Biology at Linfield University, where she teaches principles of biology, genetics, molecular cell biology, and animal development courses and conducts research with undergraduates. Her research interests include deciphering molecular mechanisms of gene regulation. She earned her BA in Biology and English from Carleton College, where she studied the regulation of inflorescence development with Susan Singer, and her PhD in Molecular Genetics and Cell Biology from the University of Chicago, where she studied mechanisms of endoplasmic reticulum inheritance and Golgi biogenesis with Benjamin Glick. After her introduction to teaching at Carleton College, she completed a postdoctoral fellowship with Richard Carthew in the Department of Molecular Biosciences at Northwestern University, where she studied molecular mechanisms of microRNA-mediated gene silencing. She is a recipient of the Laurence McKinley Gould Prize in Natural Science from Carleton College. She is also an active collaborator and principal investigator in the Genomics Education Partnership. She enjoys time spent with old friends and exploring new and cherished destinations with her husband John and children, Isaac and Ezra.

John L. Tymoczko

John L. Tymoczko was Towsley Professor of Biology Emeritus at Carleton College, where he taught from 1976 until his death in 2019. He taught a variety of courses, including Biochemistry, Biochemistry Laboratory, Oncogenes and the Molecular Biology of Cancer, and Exercise Biochemistry, and cotaught an introductory course, Energy Flow in Biological Systems. Professor Tymoczko received his B.A. from the University of Chicago in 1970 and his PhD in Biochemistry from the University of Chicago with Shutsung Liao at the Ben May Institute for Cancer Research. He then had a postdoctoral position with Hewson Swift of the Department of Biology at the University of Chicago. The focus of his research was on steroid receptors, ribonucleoprotein particles, and proteolytic processing enzymes.