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Physical Biochemistry
Second Edition©1983 David M. Freifelder
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ISBN:9780716714446
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Suitable for advanced undergraduate and graduate students in biochemistry, this book provides clear, concise, well-exampled descriptions of the physical methods that biochemists and molecular biologists use.
Table of Contents
I. CHARACTERIZATION OF MACROMOLECULES
1. Direct Observation
2. Light Microscopy
3. Electron Microscopy
II. GENERAL LABORATORY METHODS
4. Measurement of pH
5. Radioactive Labeling and Counting
6. Autoradiography
7. Membrane Filtration and Dialysis
III. SEPARATION AND IDENTIFICATION OF MATERIALS
8. Chromatography
9. Electrophoresis
10. Immunological Methods
IV. HYDRODYNAMIC METHODS
11. Sedimentation
12. Partial Specific Volume and the Diffusion Coefficient
13. Viscosity
V. SPECTROSCOPIC METHODS
14. Absorption Spectroscopy
15. Fluorescence Spectroscopy
16. Optical Rotary Dispersion and Circular Dichroism
17. Nuclear Magnetic Resonance and Electron Spin Resonance
VI. MISCELLANEOUS METHODS
18. Ligand Binding
19. Miscellaneous Methods
Answers to Problems
1. Direct Observation
2. Light Microscopy
3. Electron Microscopy
II. GENERAL LABORATORY METHODS
4. Measurement of pH
5. Radioactive Labeling and Counting
6. Autoradiography
7. Membrane Filtration and Dialysis
III. SEPARATION AND IDENTIFICATION OF MATERIALS
8. Chromatography
9. Electrophoresis
10. Immunological Methods
IV. HYDRODYNAMIC METHODS
11. Sedimentation
12. Partial Specific Volume and the Diffusion Coefficient
13. Viscosity
V. SPECTROSCOPIC METHODS
14. Absorption Spectroscopy
15. Fluorescence Spectroscopy
16. Optical Rotary Dispersion and Circular Dichroism
17. Nuclear Magnetic Resonance and Electron Spin Resonance
VI. MISCELLANEOUS METHODS
18. Ligand Binding
19. Miscellaneous Methods
Answers to Problems
David M. Freifelder
David M. Freifelder was Professor of Biochemistry at Brandeis Unversity. He received his doctorate from the University of Chicago. Professor Freifelder has also been a visiting scholar at the Weizman Institute and Stanford University.