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Cover: Achieve Labs for Microbiology Simulations (1-Term Access), 1st Edition by Macmillan Learning
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Achieve Labs for Microbiology Simulations (1-Term Access)

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First  Edition|©2022  Macmillan Learning

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ISBN:9781319430290

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  • About
  • Digital Options
  • Contents
  • Authors

About

Microbiology Labs from the comfort of your own home

These open-ended lab simulations provide the most realistic virtual lab experience available. Access any lab exercise you’ll need for microbiology at any time, and complete the work at your own pace. Targeted hints, feedback, and solutions ensure you’re on track and understand the materials.

Digital Options

Achieve

Achieve is a single, easy-to-use platform proven to engage students for better course outcomes

Learn More

Contents

Table of Contents

Achieve Lab Simulations for Microbiology
Brief Table of Contents

Introduction
Goal Setting and Reflection Surveys
Welcome to Achieve

Lab Safety

  • Experiment 1: Safety Features and Hazards
  • Experiment 2: Dressing for the Lab

Introduction to Lab Simulations

  • Experiment 1: Measure a Change in Temperature Following a Chemical Reaction Between HCl and NaOH
  • Experiment 2: Investigate the Relationship between the Volume and Pressure of a Gas (includes Smart Worksheet)
  • Experiment 3: Observing a Reaction Between HCl and Zinc

Scientific Method

  • Experiment 1: Find the Best Mouse Diet for Weight Gain (includes Smart Worksheet)
  • Experiment 2: Replicate the Experiment (includes Smart Worksheet)

Basic Microscopy

  • Experiment 1: Visualizing Cells Using a Microscope
  • Experiment 2: Estimating Size

Aseptic Technique

  • Experiment 1: Pouring an Agar Plate
  • Experiment 2: Making a Streak Plate Using the Aseptic Technique

Cultivation of Bacteria

  • Experiment 1: Bacteria in the Environment
  • Experiment 2: Isolation of Bacteria

Staining

  • Experiment 1: Gram Staining
  • Experiment 2: Acid-Fast Staining
  • Experiment 3: Capsule Staining of K. pneumoniae and S. pneumoniae

Enumeration of Bacteria

  • Experiment 1: Spread Plating of Bacterial Serial Dilutions (includes Smart Worksheet)
  • Experiment 2: Counting Plate CFUs (includes Smart Worksheet)
  • Experiment 3: Calculating Sample CFU Density (includes Smart Worksheet)

Motility and Amino Acid Hydrolysis

  • Experiment 1: Motility
  • Experiment 2: Amino Acid Hydrolysis

Blood

  • Experiment 1: Normal Peripheral Blood (includes Smart Worksheet)
  • Experiment 2: Diseased Peripheral Blood (includes Smart Worksheet)

Unknown Identification

  • Experiment 1: Gram Stain
  • Experiment 2: Starch Hydrolysis Test
  • Experiment 3: Citrate Test
  • Experiment 4: Anaerobic Jar

Control of Bacterial Growth

  • Experiment 1: UV Radiation
  • Experiment 2: Chemical Agent Effects on E. coli
  • Experiment 3: Pressurized Steam, High-Temperature Sterilization Using an Autoclave

Bacterial Transformation

  • Experiment 1: Bacterial Transformation with an Ampicillin-Resistant Plasmid (includes Smart Worksheet)
  • Experiment 2: Determining Transformation Success Using Selective Media (includes Smart Worksheet)

Extreme Bacteria

  • Experiment 1: Effect of Temperature on Microbial Growth
  • Experiment 2: Effect of pH on Microbial Growth
  • Experiment 3: Effect of Osmotic Pressure on Microbial Growth
  • Experiment 4: Effect of Oxygen on Microbial Growth

Antibiotic Sensitivity

  • Experiment 1: Performing a Disk Diffusion Test with E. coli
  • Experiment 2: Performing a Disk Diffusion Test with S. aureus
  • Experiment 3: Performing a Disk Diffusion Test with MRSA

Bacteria

  • Experiment 1: Performing Gram Staining of S. aureus and E. coli
  • Experiment 2: Determining the Susceptibility of Gram-Positive and Gram-Negative Bacteria to Antibiotics

Protists

  • Topic 1: Investigating Excavata
  • Topic 2: Investigating Rhizaria
  • Topic 3: Investigating Chromalveolata
  • Topic 4: Investigating Archaeplastida
  • Topic 5: Investigating Amoebas
  • Topic 6: Investigating Choanoflagellates

Fungi

  • Topic 1: Investigating Phylum Basidiomycota
  • Topic 2: Investigating Phylum Ascomycota
  • Topic 3: Investigating Phylum Zygomycota

Acids, Bases, and pH Buffers

  • Experiment 1: Measuring pH by Using the pH Indicator Bromothymol Blue (includes Smart Worksheet)
  • Experiment 2: The Phosphate Buffer System (includes Smart Worksheet)
  • Experiment 3: Measuring the Buffer Capacity of a Phosphate Buffer (includes Smart Worksheet)

Biological Molecules

  • Experiment 1: Testing for Reducing Sugars Using Benedict’s Solution
  • Experiment 2: Testing for Starch Using Lugol’s Iodine
  • Experiment 3: Testing for Lipids Using Sudan III Solution
  • Experiment 4: Testing for Proteins Using Biuret Solution
  • Experiment 5: Testing Various Foods for Reducing Sugars, Starch, Lipids, and Proteins

Enzymes

  • Experiment 1: Determining the Effect of Temperature on Catalase Activity (includes Smart Worksheet)
  • Experiment 2: Determining the Effect of Substrate Concentration on Catalase Activity (includes Smart Worksheet)
  • Experiment 3: Determining the Effect of pH on Catalase Activity (includes Smart Worksheet)

Quantitative Analysis of Enzyme Activity

  • Experiment 1: Creating a Calibration Curve for Starch–Iodine Measurements (includes Smart Worksheet)
  • Experiment 2: Determining the Effect of pH on Amylase Enzyme Activity (includes Smart Worksheet)
  • Experiment 3: Determining the Effect of Temperature on Amylase Enzyme Activity (includes Smart Worksheet)

Diffusion and Osmosis

  • Experiment 1: Qualitative Evidence for Diffusion
  • Experiment 2: Quantifying the Relationship Between Concentration Gradient and Osmosis (includes Smart Worksheet)
  • Experiment 3: Visualizing Osmosis in Living Cells

Expanded Diffusion and Osmosis

  • Experiment 1: Qualitative Evidence for Diffusion
  • Experiment 2: Observing the Dependence of the Rate of Diffusion on the Concentration Gradient (includes Smart Worksheet)
  • Experiment 3: Confirming Osmosis by Quantifying Weight Changes and Screening for Protein
  • Experiment 4: Quantifying the Relationship Between Concentration Gradient and Osmosis (includes Smart Worksheet)

Cellular Respiration

  • Experiment: Fermentation of Different Sugars by Yeast Cells (includes Smart Worksheet)

DNA

  • Experiment: Running a Gel Electrophoresis of DNA VNTR Fragments (includes Smart Worksheet)

Regulation of Gene Expression

  • Topic 1: Investigating Gene Expression
  • Topic 2: Investigating mRNA Production
  • Topic 3: Optimizing Protein Production Using Multiple Cells

PCR

  • Experiment 1: Running a PCR
  • Experiment 2: Conducting Gel Electrophoresis

Nucleic Acid Assays

  • Experiment 1: Performing an RNA Extraction from Animal Cells
  • Experiment 2: Cloning
  • Experiment 3: Next Generation Sequencing

Authors

Macmillan Learning


Achieve More with Microbiology Lab Simulations  2.0

Microbiology Labs from the comfort of your own home

These open-ended lab simulations provide the most realistic virtual lab experience available. Access any lab exercise you’ll need for microbiology at any time, and complete the work at your own pace. Targeted hints, feedback, and solutions ensure you’re on track and understand the materials.

Achieve

Achieve is a single, easy-to-use platform proven to engage students for better course outcomes

Learn More

Table of Contents

Achieve Lab Simulations for Microbiology
Brief Table of Contents

Introduction
Goal Setting and Reflection Surveys
Welcome to Achieve

Lab Safety

  • Experiment 1: Safety Features and Hazards
  • Experiment 2: Dressing for the Lab

Introduction to Lab Simulations

  • Experiment 1: Measure a Change in Temperature Following a Chemical Reaction Between HCl and NaOH
  • Experiment 2: Investigate the Relationship between the Volume and Pressure of a Gas (includes Smart Worksheet)
  • Experiment 3: Observing a Reaction Between HCl and Zinc

Scientific Method

  • Experiment 1: Find the Best Mouse Diet for Weight Gain (includes Smart Worksheet)
  • Experiment 2: Replicate the Experiment (includes Smart Worksheet)

Basic Microscopy

  • Experiment 1: Visualizing Cells Using a Microscope
  • Experiment 2: Estimating Size

Aseptic Technique

  • Experiment 1: Pouring an Agar Plate
  • Experiment 2: Making a Streak Plate Using the Aseptic Technique

Cultivation of Bacteria

  • Experiment 1: Bacteria in the Environment
  • Experiment 2: Isolation of Bacteria

Staining

  • Experiment 1: Gram Staining
  • Experiment 2: Acid-Fast Staining
  • Experiment 3: Capsule Staining of K. pneumoniae and S. pneumoniae

Enumeration of Bacteria

  • Experiment 1: Spread Plating of Bacterial Serial Dilutions (includes Smart Worksheet)
  • Experiment 2: Counting Plate CFUs (includes Smart Worksheet)
  • Experiment 3: Calculating Sample CFU Density (includes Smart Worksheet)

Motility and Amino Acid Hydrolysis

  • Experiment 1: Motility
  • Experiment 2: Amino Acid Hydrolysis

Blood

  • Experiment 1: Normal Peripheral Blood (includes Smart Worksheet)
  • Experiment 2: Diseased Peripheral Blood (includes Smart Worksheet)

Unknown Identification

  • Experiment 1: Gram Stain
  • Experiment 2: Starch Hydrolysis Test
  • Experiment 3: Citrate Test
  • Experiment 4: Anaerobic Jar

Control of Bacterial Growth

  • Experiment 1: UV Radiation
  • Experiment 2: Chemical Agent Effects on E. coli
  • Experiment 3: Pressurized Steam, High-Temperature Sterilization Using an Autoclave

Bacterial Transformation

  • Experiment 1: Bacterial Transformation with an Ampicillin-Resistant Plasmid (includes Smart Worksheet)
  • Experiment 2: Determining Transformation Success Using Selective Media (includes Smart Worksheet)

Extreme Bacteria

  • Experiment 1: Effect of Temperature on Microbial Growth
  • Experiment 2: Effect of pH on Microbial Growth
  • Experiment 3: Effect of Osmotic Pressure on Microbial Growth
  • Experiment 4: Effect of Oxygen on Microbial Growth

Antibiotic Sensitivity

  • Experiment 1: Performing a Disk Diffusion Test with E. coli
  • Experiment 2: Performing a Disk Diffusion Test with S. aureus
  • Experiment 3: Performing a Disk Diffusion Test with MRSA

Bacteria

  • Experiment 1: Performing Gram Staining of S. aureus and E. coli
  • Experiment 2: Determining the Susceptibility of Gram-Positive and Gram-Negative Bacteria to Antibiotics

Protists

  • Topic 1: Investigating Excavata
  • Topic 2: Investigating Rhizaria
  • Topic 3: Investigating Chromalveolata
  • Topic 4: Investigating Archaeplastida
  • Topic 5: Investigating Amoebas
  • Topic 6: Investigating Choanoflagellates

Fungi

  • Topic 1: Investigating Phylum Basidiomycota
  • Topic 2: Investigating Phylum Ascomycota
  • Topic 3: Investigating Phylum Zygomycota

Acids, Bases, and pH Buffers

  • Experiment 1: Measuring pH by Using the pH Indicator Bromothymol Blue (includes Smart Worksheet)
  • Experiment 2: The Phosphate Buffer System (includes Smart Worksheet)
  • Experiment 3: Measuring the Buffer Capacity of a Phosphate Buffer (includes Smart Worksheet)

Biological Molecules

  • Experiment 1: Testing for Reducing Sugars Using Benedict’s Solution
  • Experiment 2: Testing for Starch Using Lugol’s Iodine
  • Experiment 3: Testing for Lipids Using Sudan III Solution
  • Experiment 4: Testing for Proteins Using Biuret Solution
  • Experiment 5: Testing Various Foods for Reducing Sugars, Starch, Lipids, and Proteins

Enzymes

  • Experiment 1: Determining the Effect of Temperature on Catalase Activity (includes Smart Worksheet)
  • Experiment 2: Determining the Effect of Substrate Concentration on Catalase Activity (includes Smart Worksheet)
  • Experiment 3: Determining the Effect of pH on Catalase Activity (includes Smart Worksheet)

Quantitative Analysis of Enzyme Activity

  • Experiment 1: Creating a Calibration Curve for Starch–Iodine Measurements (includes Smart Worksheet)
  • Experiment 2: Determining the Effect of pH on Amylase Enzyme Activity (includes Smart Worksheet)
  • Experiment 3: Determining the Effect of Temperature on Amylase Enzyme Activity (includes Smart Worksheet)

Diffusion and Osmosis

  • Experiment 1: Qualitative Evidence for Diffusion
  • Experiment 2: Quantifying the Relationship Between Concentration Gradient and Osmosis (includes Smart Worksheet)
  • Experiment 3: Visualizing Osmosis in Living Cells

Expanded Diffusion and Osmosis

  • Experiment 1: Qualitative Evidence for Diffusion
  • Experiment 2: Observing the Dependence of the Rate of Diffusion on the Concentration Gradient (includes Smart Worksheet)
  • Experiment 3: Confirming Osmosis by Quantifying Weight Changes and Screening for Protein
  • Experiment 4: Quantifying the Relationship Between Concentration Gradient and Osmosis (includes Smart Worksheet)

Cellular Respiration

  • Experiment: Fermentation of Different Sugars by Yeast Cells (includes Smart Worksheet)

DNA

  • Experiment: Running a Gel Electrophoresis of DNA VNTR Fragments (includes Smart Worksheet)

Regulation of Gene Expression

  • Topic 1: Investigating Gene Expression
  • Topic 2: Investigating mRNA Production
  • Topic 3: Optimizing Protein Production Using Multiple Cells

PCR

  • Experiment 1: Running a PCR
  • Experiment 2: Conducting Gel Electrophoresis

Nucleic Acid Assays

  • Experiment 1: Performing an RNA Extraction from Animal Cells
  • Experiment 2: Cloning
  • Experiment 3: Next Generation Sequencing
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